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Correlation between macrophage migration inhibitory factor and autophagy in Helicobacter pylori-associated gastric

Kichul Yoon1, Nayoung Kim1,2, Youngmi Park3

  • 1Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, South Korea.

Plos One
|February 12, 2019
PubMed

Insights

Macrophage migration inhibitory factor (MIF) and autophagy markers play a role in H. pylori-associated gastric cancer. Their interaction suggests a link to gastric carcinogenesis.

Area of Science:

  • Gastroenterology
  • Oncology
  • Molecular Biology

Background:

  • The roles of macrophage migration inhibitory factor (MIF) and autophagy in gastric cancer remain unclear.
  • Helicobacter pylori (H. pylori) infection is a known risk factor for gastric cancer.
  • Autophagy markers include Atg5, LC3A, and LC3B.

Purpose of the Study:

  • To investigate the expression of MIF and autophagy markers in relation to H. pylori infection and gastric carcinogenesis.
  • To explore the interaction between MIF and autophagy markers in H. pylori-associated gastric cancer.

Main Methods:

  • Real-time PCR was used to measure mRNA expression of MIF, Atg5, LC3A, and LC3B in 453 patients (control, gastric dysplasia, gastric cancer).
  • H. pylori infection status was determined, and eradication therapy was performed for positive patients.
  • MIF and Atg5 levels were monitored during follow-up in H. pylori-eradicated and H. pylori-negative groups.
  • Multiple linear regression analysis was employed to assess the predictive value of autophagy markers on MIF levels.

Main Results:

  • In H. pylori-positive patients, MIF levels were significantly elevated in the cancer group compared to control or dysplasia groups.
  • Autophagy markers LC3A and LC3B showed significant differences within H. pylori-positive subgroups.
  • Atg5 expression increased sequentially from control to cancer subgroups in the H. pylori-eradicated group.
  • Autophagy markers (LC3A, LC3B, Atg5) were direct predictors of MIF levels (adjusted R2 = 0.492, P<0.001).
  • H. pylori-eradicated patients consistently showed higher Atg5 levels than H. pylori-negative patients during follow-up.

Conclusions:

  • MIF and autophagy markers, particularly in the context of H. pylori infection, are implicated in gastric carcinogenesis.
  • The interaction between MIF and autophagy markers appears to be a significant factor in the development of gastric cancer.
  • Further research into these molecular pathways could lead to novel diagnostic or therapeutic strategies for H. pylori-associated gastric cancer.

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