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Purification and characterization of native human elongation factor 2
Rasmus Kock Flygaard1, Beatrice Malacrida2, Patrick Kiely2
1Department of Molecular Biology and Genetics, University of Aarhus, Denmark.
Abstract:
Human elongation factor 2 is the translocase that is responsible for the movement of tRNA from the A- to P- and P- to E-site on the ribosome during the elongation phase of translation. Being a vital factor of protein biosynthesis, its function is highly controlled and regulated. It has been implicated in numerous diseases and pathologies, and as such it is important to have a source for isolated pure and active protein for biomedical and biochemical studies. Here we report development of a purification protocol for native human elongation factor 2 from HEK-293S cells. The resulting protein is active, pure, has an intact diphtamide and is obtainable in yields suitable for functional and structural studies.
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