Related Experiment Video
Updated: Jan 29, 2026
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
Published on: July 6, 2012
Multiplexing protein and gene level measurements on a single Luminex platform
Damon B Cook1, Brian C McLucas1, Leticia A Montoya1
1Thermo Fisher Scientific, Carlsbad, CA 92008, United States.
Background:
The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional immunoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.
Method And Results:
Here, we demonstrate two high-throughput assays measuring both messenger RNA (mRNA) expression and proteins in a single sample run on a Luminex platform. Human peripheral blood mononuclear cells (hPBMCs) were treated with lipopolysaccharide (LPS) and harvested at 24 and 72 h. Samples were assayed with the ProcartaPlex Human Immune Monitoring 65-plex Panel for protein and corresponding mRNA targets on a QuantiGene Human 80-plex Panel.
Conclusion:
Multiplexing ProcartaPlex and QuantiGene Plex assays provide a broad survey of protein levels and gene expression networks.
More Related Videos
10:37Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
07:08A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
Published on: April 6, 2021
Related Concept Videos
02:11Proteins: From Genes to Degradation
Transcription is the synthesis of RNA...
02:11Proteins: From Genes to Degradation
00:54Ratio Level of Measurement
A set of data measured using the ratio scale takes care of the ratio problem and provides complete information. Ratio scale data are like interval scale data, except they have a zero point and ratios can be calculated....
00:55Ordinal Level of Measurement
Data measured using an ordinal scale are similar to nominal scale data, but there is one major difference. The ordinal scale data can be ordered. An example of ordinal scale data is a list of the top five national parks...
00:55Interval Level of Measurement
Data measured using the interval scale are similar to ordinal level data because they have a definite arrangement. However, in the interval level of measurement, the differences between data values are meaningful even though the data does not have a starting point.
Temperature is measured using the interval scale. It is measurable data, and the difference between...
00:56Nominal Level of Measurement
The data that cannot be measured but can be grouped into categories fall under the nominal level of measurement. Data that is measured using a nominal...