Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Weir01:24

Weir

500
A weir is a hydraulic structure designed to partially obstruct an open channel, enabling precise control and measurement of water flow. By forcing water to flow over or through it, a weir allows for accurate determination of discharge rates, making it an essential tool in water resource management. These structures are extensively used in regulating river flows, irrigation systems, and flood control channels.Types of Weirs and Their FeaturesWeirs are categorized primarily into sharp-crested and...
500
Slant Asymptotes01:27

Slant Asymptotes

119
A function's behavior is often guided by asymptotic constraints, where one term dominates another, defining a limiting trend. In the given scenario, the mathematical pattern follows a rational function: a cubic term in the numerator is divided by a squared term in the denominator. This results in a function with distinct characteristics, including an oblique asymptote, critical points, and undefined regions.The function's validity is determined by the denominator, which must be nonzero. This...
119
Weir: Problem Solving01:26

Weir: Problem Solving

511
Water flow in open channels is often measured using hydraulic structures such as weirs, which allow precise calculation of discharge. In a rectangular channel, flow rates are measured using three types of weirs: rectangular sharp-crested, triangular sharp-crested, and broad-crested. The weir head is set at a fixed height above the channel bottom, simplifying calculations and enabling the relationship between depth and flow rate to be analyzed.For the rectangular sharp-crested weir, the flow...
511
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

7.5K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.5K
Coronary Circulation01:21

Coronary Circulation

7.2K
The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
7.2K
Fetal Circulation01:14

Fetal Circulation

2.9K
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical Proof of Concept for the Single-Protein Anticancer Molecule Targeting Both a Tumor Surface Antigen and an Intracellular Oncoprotein.

Advanced healthcare materials·2025
Same author

Adipocyte-derived apelin protects against radiation-induced enteropathy through Wnt/β-catenin-mediated intestinal regeneration.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Effects of Hydrolysis Reaction and Abrasive Drag Force Accelerator on Enhancing Si-Wafer Polishing Rate and Improving Si-Wafer Surface Roughness.

Nanomaterials (Basel, Switzerland)·2025
Same author

Bixin alleviates radiation-induced intestinal damage <i>via</i> inflammation regulation and barrier recovery.

International journal of radiation biology·2025
Same author

Establishment of an <i>in vitro</i> erythroid differentiation system from canine peripheral blood mononuclear cells.

Animal cells and systems·2025
Same author

The Chemical Deformation of a Thermally Cured Polyimide Film Surface into Neutral 1,2,4,5-Benzentetracarbonyliron and 4,4'-Oxydianiline to Remarkably Enhance the Chemical-Mechanical Planarization Polishing Rate.

Nanomaterials (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jan 29, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.7K

Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device.

Yousang Yoon1, Jusin Lee2, Moonsoo Ra3

  • 1Department of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea. ysyoon88@hanyang.ac.kr.

Cancers
|February 13, 2019
PubMed
Summary

This study introduces a microfluidic device for efficient separation of circulating tumor cells (CTCs) from blood. The technology shows high potential for cancer research and liquid biopsy applications.

Keywords:
cell separationcirculating tumor cellmicrofluidics

More Related Videos

Capture and Release of Viable Circulating Tumor Cells from Blood
08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

Published on: October 28, 2016

9.0K
Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

12.1K

Related Experiment Videos

Last Updated: Jan 29, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.7K
Capture and Release of Viable Circulating Tumor Cells from Blood
08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

Published on: October 28, 2016

9.0K
Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

12.1K

Area of Science:

  • Biomedical Engineering
  • Oncology
  • Microfluidics

Background:

  • Circulating tumor cells (CTCs) are crucial biomarkers for cancer detection and monitoring.
  • Existing CTC separation methods face limitations due to tumor heterogeneity and reliance on specific markers or size alone.

Purpose of the Study:

  • To develop and validate a novel microfluidic device for efficient CTC separation from unprocessed whole blood.
  • To leverage cell size and deformability for CTC isolation, overcoming limitations of current techniques.

Main Methods:

  • A slanted weir microfluidic device was designed and utilized.
  • The device separates cells based on size and deformability, processing whole blood.
  • An integrated image analysis tool was developed for cell characterization.

Main Results:

  • Achieved 97% separation efficiency for CTCs using a highly invasive breast cancer cell line.
  • Demonstrated significant depletion of erythrocytes (8-log) and leukocytes (5.6-log).
  • The image analysis tool successfully characterized cell morphology and deformability.

Conclusions:

  • The developed microfluidic system demonstrates high potential for effective CTC isolation.
  • This technology could significantly advance liquid biopsy applications and cancer research.