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Published on: December 5, 2013
Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro - an edible fern
R C Thakur1, Y Hosoi1, K Ishii1
1Bio-Resources Technology Division, Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norinkenkyu Danchi-nai, Ibaraki 305, Japan Fax: +81-298-73-3795 e-mail: thakur@ffpri.affrc.go.jp, , , , , , JP.
Abstract:
A rapid and efficient in vitro plant regeneration method was developed for Matteuccia struthiopteris (L.) Todaro (Ostrich fern). Side shoots, originating in meristems of sectioned rhizomes, were used as explant material. A very high rate of meristem multiplication was achieved by culturing the explants in half-strength MS liquid medium supplemented with 2.0 mg/l N-(4-Pyridyl)-N'-phenylurea (4-PU) and 0.5 mg/l thidiazuron (TDZ). Multiplication of the shoot primordia was faster in suspension culture than on solid medium. Rhizogenesis and growth of regenerants were best achieved on hormone-free one-quarter-strength MS solid medium amended with 0.4% agar and 1.0% activated charcoal. Regenerated plantlets continued to grow after transfer to soil in a phytotron.
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