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Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
Isolation and identification of littorine from hairy roots of Atropa belladonna
F Nakanishi1, K Sasaki2, K Shimomura3
1Department of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan Fax: +81-423-29 7737 e-mail: fuminaka@u-gakugei.ac.jp, , , , , , JP.
Abstract:
A hairy root clone (M8) of Atropa belladonna, producing high levels of tropane alkaloids, was established by transformation with Agrobacterium rhizogenes (MAFF 03-01724). Littorine, an intermediate of tropane alkaloids, was detected by high-performance liquid chromatography and gas chromatography-mass spectrometry in the alkaloid fraction of the hairy roots and identified by nuclear magnetic resonance analysis. Littorine was also detected in the non-transformed root culture of A. belladonna.
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