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A non-destructive selection method for resistance to fusicoccin in Arabidopsis thaliana
I Murgia1, P Morandini1, A Moroni1
1Dipartimento di Biologia "L. Gorini", Sezione di Fisiologia e Biochimica delle Piante, via Celoria 26, I-20133 Milan, Italy e-mail: Carlo.Soave@unimi.it Fax: +39-2-26604399, , , , , , IT.
Abstract:
A mutagenised population of seeds of Arabidopsis thaliana was allowed to germinate in the presence of the positively charged aminoglycoside hygromycin (4 μg/ml) and the fungal toxin fusicoccin (5×10-6 M). This hygromycin concentration, which is non-toxic by itself, becomes toxic when used together with fusicoccin, which stimulates cation uptake. Seeds that had germinated after 3-5 days and produced seedlings with green cotyledons were potentially resistant to fusicoccin and were therefore transferred into sterile Magenta vessels containing 1/2-strength Murashige and Skoog medium. This selection procedure is non-destructive, i.e. it allows the recovery of viable seedlings and their growth into adult plants thus permitting direct physiological characterisation.
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