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Preparation process optimization of pig bone collagen peptide-calcium chelate using response surface methodology and
Wenmin Wu1, Lichao He2, Yanhui Liang1
1College of Food Science and Technology & MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Food Chemistry
|February 13, 2019
Summary
This study developed a collagen peptide-calcium chelate with high calcium binding ability using alcalase and neutrase. The resulting chelate enhances calcium absorption in the gastrointestinal tract and Caco-2 cells.
Area of Science:
- Biochemistry
- Nutritional Science
- Biotechnology
Background:
- Collagen peptides are recognized for their potential bioactivity.
- Enhancing calcium bioavailability is crucial for bone health.
- Developing novel calcium supplements with improved absorption is an ongoing research area.
Purpose of the Study:
- To prepare collagen peptides with high calcium binding ability.
- To optimize the conditions for peptide-calcium chelate formation.
- To investigate the stability and calcium absorption-promoting effects of the chelate.
Main Methods:
- Enzymatic hydrolysis of collagen using alcalase and neutrase.
- Response surface methodology (RSM) for optimizing chelate preparation.
- Spectroscopic analysis (UV-Vis, fluorescence, FT-IR) to characterize the chelate.
- In vitro Caco-2 cell monolayer model to assess calcium transport.
Main Results:
- Optimal conditions yielded a calcium chelating rate of 78.38%.
- Spectroscopic data confirmed calcium chelation by collagen peptide functional groups.
- The peptide-calcium chelate demonstrated stability across various temperatures, pH, and simulated gastrointestinal conditions.
- Significant enhancement of calcium transport in Caco-2 cells was observed, with reversal of inhibition by phosphate and phytate.
Conclusions:
- A stable and effective peptide-calcium chelate was successfully prepared.
- The chelate promotes calcium absorption and offers potential for new calcium supplements.
- This research supports the high-value utilization of pig bone collagen.
Keywords:
AbsorptionCaco-2 cell monolayer modelCalcium dichloride (PubChem CID: 5284359)Calcium-binding peptideCholecalcifetrol (PubChem CID: 5280795)Citric acid (PubChem CID: 311)Ethanol (PubChem CID: 702)Formaldehyde (PubChem CID: 712)Hexane (PubChem CID: 8058)Peptide-calcium chelatePig bonePotassium Phosphate, Monobasic (PubChem CID: 516951)Response surface methodologySodium hydroxide (PubChem CID: 14798)Sodium phytate (PubChem CID: 66391)Structural characterizationTrichloroacetic acid (PubChem CID: 6421)More Related Videos
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