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Updated: Jan 29, 2026

Decoding Natural Behavior from Neuroethological Embedding
Published on: October 3, 2025
Jaburetox, a natural insecticide derived from Jack Bean Urease, activates voltage-gated sodium channels to modulate
Douglas Silva Dos Santos1, Ana Paula Zanatta2, Anne Helene Souza Martinelli3
1Graduate Program in Biological Sciences: Biochemical Toxicology, Federal University of Santa Maria-UFSM, Santa Maria, RS, Brazil; University of Toronto Mississauga-UTM, Department of Biology. Mississauga, ON, Canada.
Abstract:
Jaburetox (Jbtx) is an insecticidal peptide derived from Canavalia ensiformis urease, whose mechanism of action is not completely elucidated. We employed behavioral, electromyographical and electrophysiological protocols to identify the cellular and molecular targets involved in the Jbtx entomotoxicity in cockroaches and locusts. In Nauphoeta cinerea, Jbtx (32 μg/g) altered the locomotory behaviour inducing a significative decrease in the distance travelled followed by a significant increase in stopped time (52 ± 85 cm and 2573 ± 89 s, p < .05, n = 40). Jbtx (8 to 32 μg/g body weight, respectively) also increased the leg and antennae grooming activities (p < .05, n = 40, respectively). Jbtx (8 to 16 μg/g) induced a maximum neuromuscular blockade of 80.72% (n = 6, p < .05) and was cardiotoxic, decreasing the cockroach heart rate. The electrophysiological profiles of both muscle and nerve of L. migratoria showed that Jbtx (2.5 × 10-7 and 2.5 × 10-3 μg/ body weight) induced a significant increase in the amplitude of nerve action potentials (n = 5, p < .05). Voltage clamp analysis of Jbtx (200 nM) applied in Xenopus laevis oocytes heterologously expressed with Nav 1.1 channels showed a significant increase in the sodium currents. In conclusion, this work revealed that the entomotoxic activity of Jbtx involves complex behavioral alterations that begins with an initial activation of voltage-gated sodium channels.
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