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Updated: Jan 29, 2026

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
Published on: February 13, 2012
Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic
Yuan Gao1, Ying Mao1, Rong-Gang Xu2
1PKU-THU Joint Center for Life Sciences, College of Life Sciences, School of Medicine, Tsinghua University, Beijing, 100084, China.
Abstract:
In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study, we identified 173 EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.
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