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Large area sectioning for morphologic studies of nonhuman primate nasal cavities
H W Randall1, T M Monticello, K T Morgan
1Department of Experimental Pathology and Toxicology, Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
Stain Technology
|November 1, 1988
Summary
Histologic preparation of primate nasal cavities is challenging. This new method provides complete, undecalcified transverse sections for inhalation toxicology and autoradiographic studies.
Area of Science:
- Comparative Anatomy
- Histology
- Toxicology
Background:
- The nasal region is crucial for inhalation toxicology studies.
- Histological preparation of primate nasal cavities presents significant challenges due to their size.
- Existing methods may not yield complete or detailed sections.
Purpose of the Study:
- To describe a novel method for histological preparation of undecalcified, complete transverse sections of the nonhuman primate nasal cavity.
- To facilitate detailed morphological examination and advanced studies like autoradiography.
Main Methods:
- Heads were fixed in formalin after removing excess tissue, mandible, and calvaria.
- Nasal regions were serially sectioned into 3-mm blocks using a water-cooled diamond blade.
- Blocks were embedded in a glycol and methyl methacrylate mixture, sectioned at 2-4 microns.
Main Results:
- The technique produced complete transverse sections of nasal cavities with excellent morphological detail.
- Sections were suitable for various staining procedures and autoradiography.
- The embedding mixture was effective for undecalcified bone and teeth.
Conclusions:
- This method offers a reliable approach for histological examination of primate nasal cavities.
- It enables detailed studies in inhalation toxicology and related research areas.
- The technique is advantageous for preserving fine morphological structures.