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Updated: Jan 29, 2026

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Published on: May 19, 2010
The precursor osteoblast-like cell, MC3T3-E1 cell line, enhances sodium-calcium exchanger 1 (Ncx1) gene expression by
Katsuhiko Warita1,2, Ryo Aoki1, Naoki Kitamura2,3
1Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori 680-8553, Japan.
Abstract:
This study evaluated the expression of genes involved in the concentration of Ca2+ in precursor osteoblast-like cell, MC3T3-E1 subjected to stretching stimuli. Transient receptor potential vanilloid 4 (Trpv4) gene expression, the factor that is activated by stretch stimulation and enables inflow of Ca2+ from the extracellular space, was not affected as a result of stretch stimulation; conversely, the expression of sodium-calcium exchanger 1 (Ncx1) gene involved in outflow of intracellular Ca2+ increased, depending on stimulation intensity. Localization of Ca2+ correlated with the positioning of the endoplasmic reticulum, and intracellular Ca2+ decreased in inverse proportion to the intensity of the stretching force. These results suggest that stretch stimulation activates intracellular Ca2+ elimination rather than Ca2+ uptake before osteoblast differentiation.
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