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The Association between Nod2 R702w Polymorphism and Susceptibility to Colorectal Cancer in Romanian Patients.
F Burada1,2, C S Mirea3, M G Cucu2
1Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Romania.
This study found no significant association between the NOD2 R702W genetic variant and colorectal cancer (CRC) risk in the Romanian population. Further research in larger, diverse populations is needed to confirm these findings for inflammatory bowel disease (IBD) susceptibility.
Area of Science:
- Genetics
- Oncology
- Gastroenterology
Background:
- Inflammatory bowel disease (IBD) is linked to increased colorectal cancer (CRC) risk.
- NOD2 is a key susceptibility gene investigated in IBD.
- The NOD2 rs2066844 C>T (Arg702Trp/R702W) variant is of particular interest.
Purpose of the Study:
- To investigate the association between the NOD2 R702W variant and sporadic CRC risk.
- To analyze this association within a Romanian population cohort.
- To determine if this specific genetic marker influences CRC susceptibility.
Main Methods:
- A hospital-based case-control study involving 108 CRC patients and 265 controls.
- Genotyping of the NOD2 R702W variant using Real-time PCR and TaqMan assays.
- Statistical analysis of genotype and allele frequencies, calculating odds ratios (OR) and 95% confidence intervals (CI).
Main Results:
- No statistically significant difference in genotype frequencies (CC vs. CT) between CRC patients and controls (OR 1.1, p=0.83).
- No TT homozygous genotype was detected in the study population.
- No significant association was found for allele frequencies (OR 1.09, p=0.84) or in stratified analyses by tumor site, stage, or subtype.
Conclusions:
- The NOD2 R702W variant does not appear to be associated with CRC risk in the Romanian population.
- Current findings suggest this specific SNP may not be a significant risk factor for CRC in this demographic.
- Larger, multi-ethnic population studies are recommended to definitively establish the role of NOD R702W in CRC susceptibility.
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