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Updated: Jan 29, 2026

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Published on: May 3, 2013
DNA aptamer assemblies as fibroblast growth factor mimics and their application in stem cell culture
Ryosuke Ueki1, Saki Atsuta, Ayaka Ueki
1Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan. r.ueki@chembio.t.u-tokyo.ac.jp ssando@chembio.t.u-tokyo.ac.jp.
Abstract:
Replacing expensive and thermally unstable growth factors with synthetic alternatives has been an important issue in stem cell-based regenerative medicines. Here we developed DNA aptamer-assemblies that act as functional mimics of basic fibroblast growth factor (bFGF), one of the essential factors for stem cell culture. The most potent aptamer assembly named TD0, composed solely of 76-mer single-stranded DNA, could support the self-renewal and pluripotency of induced pluripotent stem cells (iPSCs). This work presents the first application of DNA aptamer in the maintenance of iPSCs.
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