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Copper nanoclusters: an efficient fluorescence sensing platform for quinoline yellow.

Unni Sivasankaran1, Jerzy Radecki2, Hanna Radecka2

  • 1Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-22, Kerala, India.

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Summary

This study developed a sensitive fluorescence sensor using l-cysteine stabilized copper nanoclusters (l-Cys-CuNCs) to detect the artificial food colorant quinoline yellow (QY). The sensor offers a low detection limit for food quality control.

Keywords:
copper nanoclustersfluorescencequinoline yellowsensorstatic quenching

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Nanotechnology

Background:

  • Artificial food colorants like quinoline yellow (QY) are widely used but require accurate detection methods.
  • Copper nanoclusters (CuNCs) exhibit unique optical properties suitable for sensing applications.
  • L-cysteine stabilization enhances the properties of copper nanoclusters.

Purpose of the Study:

  • To investigate the fluorescence quenching of quinoline yellow (QY) by l-cysteine stabilized copper nanoclusters (l-Cys-CuNCs).
  • To develop a selective and sensitive fluorescence sensor for QY detection.
  • To assess the applicability of the developed sensor in real-world food samples.

Main Methods:

  • Synthesis and characterization of l-cysteine stabilized copper nanoclusters (l-Cys-CuNCs).
  • Investigation of the fluorescence quenching mechanism upon interaction with QY.
  • Development of a fluorescence assay for QY quantification.
  • Validation of the sensor using commercial food samples.

Main Results:

  • Synthesized l-Cys-CuNCs displayed bright blue fluorescence, high quantum yield, and excellent photostability (~2 nm size).
  • The l-Cys-CuNCs selectively detected QY among other yellow colorants with a low detection limit of 0.11 μM.
  • A linear concentration range from 5.50 to 0.20 μM was established for QY detection.
  • The developed fluorescence assay demonstrated successful application in analyzing commercial food products.

Conclusions:

  • L-cysteine stabilized copper nanoclusters serve as an effective fluorescent probe for quinoline yellow detection.
  • The developed sensor provides a sensitive and selective method for QY quantification.
  • This fluorescence sensing strategy is valuable for ensuring the quality control of foodstuffs.