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Methemoglobin Forming Effect of Dimethyl Trisulfide in Mice
Márton Kiss1, Ilona Petrikovics1, David E Thompson1
1a Department of Chemistry , Sam Houston State University , Huntsville , TX , USA.
Dimethyl trisulfide (DMTS) can convert cyanide to thiocyanate. New research shows DMTS also generates methemoglobin (MetHb) in mice, suggesting a secondary therapeutic pathway for cyanide poisoning.
Area of Science:
- Toxicology
- Pharmacology
- Biochemistry
Background:
- Dimethyl trisulfide (DMTS) is a natural organic trisulfide.
- DMTS is patented as a potential antidote for cyanide (CN) poisoning.
- DMTS acts as a sulfur donor, converting CN to thiocyanate.
Purpose of the Study:
- To investigate the in vivo formation of methemoglobin (MetHb) induced by DMTS.
- To quantify DMTS-induced MetHb levels in a mouse model.
- To explore a potential secondary therapeutic mechanism of DMTS in cyanide poisoning.
Main Methods:
- Mice received intramuscular (IM) injections of formulated DMTS.
- Blood samples were collected post-injection.
- Methemoglobin (MetHb) levels were measured using spectrophotometry.
Main Results:
- DMTS administration led to a dose-dependent increase in MetHb levels in mice.
- MetHb levels peaked between 20-30 minutes post-injection.
- The highest measured MetHb levels ranged from 3.28% to 10.76% across different doses.
Conclusions:
- Intramuscular administration of DMTS induces MetHb formation in vivo.
- DMTS-generated MetHb may contribute to cyanide scavenging, indicating a secondary therapeutic pathway.
- These findings support DMTS as a multifaceted agent for cyanide poisoning treatment.
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