Enhanced mesenchymal stem cell proliferation through complexation of selenium/titanium nanocomposites
Hanaa H Ahmed1,2, Hadeer A Aglan3,4, Mostafa Mabrouk5
1Hormones Department, National Research Centre, Giza, Egypt. hanaaomr@yahoo.com.
Journal of Materials Science. Materials in Medicine
|February 13, 2019
Summary
Selenium dioxide nanoparticles and nanocomposites significantly enhanced mesenchymal stem cell proliferation. These novel nanomaterials show promise for tissue engineering applications.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue regeneration.
- Developing novel nanomaterials to modulate MSC behavior is essential for advancing regenerative medicine.
Purpose of the Study:
- To investigate the proliferative effects of selenium dioxide nanoparticles (SeO2) and selenium dioxide/titanium dioxide (Se/Ti) nanocomposites on rat bone marrow-derived MSCs (BM-MSCs) and adipose-derived stem cells (ADSCs).
- To characterize the synthesized nanomaterials using various analytical techniques.
Main Methods:
- Synthesis of SeO2 nanoparticles and Se/Ti nanocomposites via a one-step method.
- Characterization using TEM, DLS, XRD, FT-IR, SEM, and EDX.
- Isolation, propagation, and characterization of BM-MSCs and ADSCs by flow cytometry.
- Assessment of MSC proliferation using MTT assay.
Main Results:
- Nanomaterials exhibited nanoscale particle sizes.
- XRD confirmed crystalline structures, with decreasing crystallinity upon increasing TiO2 content.
- Se/Ti nanocomposites demonstrated significant MSC proliferation-promoting effects, with specific concentrations showing optimal results for ADSCs and BM-MSCs.
Conclusions:
- The synthesized SeO2 nanoparticles and Se/Ti nanocomposites effectively promote MSC proliferation.
- These nanomaterials hold significant potential for applications in tissue engineering and regenerative medicine.
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