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Updated: Jan 29, 2026

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Double-Line Reflection Pattern as a Simple Method to Determine Graft Orientation of Descemet Membrane Endothelial
Rubén D Berrospi1,2,3, Virgilio Galvis1,2,3, Maninder Bhogal4,5
1Centro Oftalmológico Virgilio Galvis, Floridablanca, Colombia.
Purpose:
To describe a simple finding, consisting of a double-line reflection from the graft inside the anterior chamber, that can be used to determine the correct donor tissue orientation in Descemet membrane endothelial keratoplasty: which we would like to call Berrospi's sign.
Methods:
Evaluation of the presence of linear reflection from the donor tissue when implanted in the anterior chamber.
Results:
A distinctive double-line reflection pattern was identified when careful observation of the curls of the scroll was performed under the surgical microscope, which was a confirmatory sign that the graft was in the correct position.
Conclusions:
This method of confirming correct graft orientation requires no additional procedures, equipment, or manipulation of the donor tissue and can be used in conjunction with other marking methods already described.
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