Related Experiment Video
Updated: Jan 29, 2026

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
Published on: March 10, 2016
Expression of avian β-defensin mRNA in the chicken yolk sac
1Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, 24061, USA.
Abstract:
The chicken yolk sac (YS) plays an important role in nutrient absorption and immune function for the developing embryo. The avian β-defensins (AvBD) are cationic peptides that are important members of the innate immune system. The objective of this study was to profile AvBD mRNA expression patterns and distribution of cells expressing AvBD mRNA in the chicken YS. Expression of AvBD1, 2, 7, and 10 mRNA was low at embryonic day 7 (e7), increased to e9 through e13 and then declined to e19. Using in situ hybridization, AvBD10 mRNA was found to be expressed in endodermal epithelial cells, while AvBD1, 2, and 7 mRNA were expressed in heterophils. The developmental expression pattern and distribution of AvBD mRNA in the YS reveals the importance of these genes to protection of the developing chick embryo.
Related Concept Videos
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression
Cell Specific Gene Expression
Regulated mRNA Transport
pre-mRNA Processing
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Nuclear Export of mRNA

