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Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus
Published on: July 31, 2021
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Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes
Lucía Casares-Crespo1, Paula Fernández-Serrano1, María P Viudes-de-Castro1
1Animal Technology and Research Center (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA), Polígono La Esperanza nº 100, 12400, Segorbe, Castellón, Spain.
Theriogenology
|February 13, 2019
Summary
Rabbit sperm proteins were characterized, revealing genotype-specific differences in abundance. This study highlights how genetic origin influences the proteome of spermatozoa, impacting reproductive processes.
Area of Science:
- Proteomics
- Animal Reproduction
- Genetics
Background:
- Spermatozoa proteome characterization is crucial for understanding male fertility.
- Genetic origin can influence physiological traits in domestic animals.
- Limited proteomic data exists for rabbit spermatozoa.
Purpose of the Study:
- To characterize rabbit sperm proteins.
- To investigate the influence of genetic origin (New Zealand White vs. California) on the rabbit sperm proteome.
- To identify differentially expressed proteins between rabbit genotypes.
Main Methods:
- Sperm protein extraction from rabbit males of two genotypes (A and R).
- In-gel digestion followed by nano liquid chromatography-tandem mass spectrometry (nano LC-MS/MS).
- Bioinformatics analysis for protein identification and abundance comparison.
Main Results:
- A library of 487 Oryctolagus cuniculus sperm proteins was identified.
- Forty proteins showed differential abundance between genotype A and genotype R.
- 25 proteins were over-expressed in genotype A, and 15 in genotype R.
Conclusions:
- This study provides the first comprehensive characterization of rabbit sperm proteins.
- Genetic origin significantly impacts the abundance of specific proteins in rabbit spermatozoa.
- These findings contribute to understanding genotype-related variations in male rabbit fertility.
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