First Report of Powdery Mildew Caused by Golovinomyces biocellatus on Peppermint in California

In August of 2009, powdery mildew was observed on peppermint (Mentha piperita L.) in several commercial fields in the Fall River Valley of eastern Shasta County, California. Plant growth was apparently reduced by the disease, but its impact on yield was unknown. White fungal growth was restricted to the adaxial surfaces, where colonies were thin and effused. Heavily infected leaves developed a reddish tint as growth prematurely ceased. Doliform conidia ([26.6-] 29.2 [-31.7] × [13.2-] 15.6 [-16.8] μm) were produced in chains of approximately six conidia. Foot cells were cylindrical ([41.3-] 55.2 [-75.0] × [11.2-] 12.0 [-12.8] μm). Immature chasmothecia were yellowish brown and approximately 100.0 μm in diameter with flexuous, mycelium-like appendages up to 200 μm long. All these features were consistent with those of Golovinomyces biocellatus. Asci were not observed. To confirm the identity of the fungus, nuclear rDNA internal transcribed spacer (ITS) regions were amplified by PCR with universal primers ITS4 and ITS5. The sequence (537 bp) was an exact match for several submissions of G. biocellatus in GenBank (e.g., Accession No. EU035602, a sequence of the fungus from mint in Australia [1]). Pathogenicity was confirmed by brushing spores from naturally infected leaves onto three rooted cuttings of M. piperita 'Black Mitchum'. After the plants were covered with a plastic bag for 36 h to maintain high humidity, they were kept on a greenhouse bench at 23 to 28°C. Three noninoculated plants, which served as controls, were placed in another greenhouse in similar conditions. The experiment was repeated once. All inoculated plants developed signs of powdery mildew within 7 days of inoculation whereas noninoculated plants remained disease free. The fungus on inoculated leaves was morphologically indistinguishable from the one used to inoculate the plants. To our knowledge, this is the first report of G. biocellatus on peppermint in California. References: (1) J. R. Liberato and J. H. Cunnington. Australas, Plant Dis. Notes 2:38, 2007.