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Updated: Jan 29, 2026

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Published on: March 10, 2020
Transgenic tomato plants expressing a Lycopersicon chilense chitinase gene demonstrate improved resistance to
Z Tabaeizadeh1, Z Agharbaoui1, H Harrak1
1Department of Biological Sciences, University of Quebec in Montreal, P.O. Box "8888," Station "Centre Ville," Montreal, Quebec, H3C 3P8, Canada e-mail: tabaeizadeh.zohreh@uqam.ca Fax: +1-514-987-4647, , , , , , CA.
Abstract:
An acidic endochitinase gene (pcht28) isolated from Lycopersicon chilense was introduced into tomato (L. esculentum) through Agrobacterium-mediated transformation, using the CAMV 35S promoter. Transgenic plants demonstrated a high level of constitutive expression of pcht28 and chitinase enzyme activity. Kanamycin-resistant R1 plants (resulting from self-pollination of transgenic plants) as well as R2 plants were evaluated for their tolerance to Verticillium dahliae (race 1 and 2 for R1 plants and race 2 for R2 plants) in the greenhouse. They demonstrated a significantly (P<0.05) higher level of tolerance to the fungi compared to the nontransgenic plants, as measured by foliar disease symptoms, vascular discoloration, and vascular discoloration index. The transgenic plants produced in this study represent a source of genetic resistance to Verticillium dahliae.
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