Related Experiment Video

Updated: Jan 29, 2026

Potato Virus X-Based microRNA Silencing VbMS In Potato.
11:51

Potato Virus X-Based microRNA Silencing VbMS In Potato.

Published on: May 11, 2020

3.6K

Sucrose utilization during potato microtuber growth in bioreactors

W-C Yu1, P J Joyce2, D C Cameron1

  • 1Department of Chemical Engineering, University of Wisconsin, Madison, WI 53706, USA, , , , , , US.

Plant Cell Reports
|February 14, 2019
PubMed

Insights

Frequent medium replacement in potato microtuber bioreactors significantly boosts growth rates and the production of larger microtubers. Optimizing sugar concentration and type is crucial for efficient sucrose utilization in seed potato production.

Area of Science:

  • Plant Biotechnology
  • Agricultural Science
  • Bioreactor Technology

Background:

  • Potato microtubers are essential for producing certified seed potatoes, serving as pathogen-tested in vitro stocks.
  • Efficient microtuber production is key to the agricultural industry.

Purpose of the Study:

  • To investigate the impact of medium refreshment frequency, sugar concentration, and sugar type on potato microtuber growth in a rotating bioreactor.
  • To identify strategies for improving sucrose utilization and microtuber yield.

Main Methods:

  • Cultivating potato microtubers in a rotating bioreactor under varying conditions.
  • Manipulating medium refreshment rates (e.g., 75% every 2 weeks vs. no refreshment).
  • Testing different sugar concentrations (40 g/L vs. 80 g/L) and types (sucrose vs. glucose/fructose mixtures).

Main Results:

  • Frequent medium replacement (75% every 2 weeks) quadrupled the number of microtubers over 1g without affecting total microtuber count.
  • Lower sugar concentration (40 g/L) and the use of glucose/fructose mixtures significantly reduced microtuber growth rates.
  • Sucrose was rapidly hydrolyzed to glucose and fructose, complicating the maintenance of optimal sucrose levels.

Conclusions:

  • Regular medium refreshment is a viable strategy to enhance the production of larger potato microtubers.
  • Optimizing sugar composition and concentration is critical for efficient microtuber development.
  • Further research into reducing sucrose hydrolysis is needed to improve bioreactor efficiency for seed potato production.
Keywords:
In vitro tuberizationInvertaseKey words Solanum tuberosum L.Micropropagation

More Related Videos

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

21.2K
Operation of a Benchtop Bioreactor12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

91.9K

Related Concept Videos

Population Growth00:57

Population Growth

Population size is dynamic, increasing with birth rates and immigration, and decreasing with death rates and emigration. In ideal conditions with unlimited resources, populations can increase exponentially, which plots as a J-shaped growth rate curve of population size against time. This type of curve is characteristic of newly-introduced invasive species, or populations that have suffered catastrophic declines and are rebounding.
28.4K
Meristems and Plant Growth02:36

Meristems and Plant Growth

Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.4K
Exponential Growth01:29

Exponential Growth

Bacterial populations exhibit exponential growth when conditions such as nutrient availability and temperature are favorable. In this phase, cells reproduce through binary fission, where each cell divides into two identical daughter cells. This process causes the population to double at regular intervals, resulting in a growth rate that is directly proportional to the current number of cells. As the population increases, the number of new cells formed during each generation also grows, creating...
50
Relationship Growth01:27

Relationship Growth

Interpersonal relationships progress through stages, beginning with awareness and moving toward mutuality, where emotional connections deepen. While many relationships remain at moderate levels of mutuality, deeper connections form through self-disclosure, trust, and interdependence.Self-DisclosureSelf-disclosure involves revealing personal information, starting with surface-level details and gradually progressing to more intimate content. As trust grows, individuals feel more comfortable...
218
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.4K
Growth versus Fixed Mindset01:24

Growth versus Fixed Mindset

Carol Dweck introduced the term mindset to describe individuals' beliefs about their intellectual and personal capabilities. These beliefs significantly influence psychological processes such as motivation, goal-setting, and perseverance, ultimately shaping academic and life outcomes. Individuals generally possess one of two mindsets- a fixed or a growth mindset—each promoting different responses to success, failure, and challenge.Fixed vs. Growth MindsetA fixed mindset assumes that one's...
296