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Updated: Jan 29, 2026

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
Published on: March 29, 2019
In vitro plant regeneration from callus of shoot apices in apple shoot culture
E Caboni1, P Lauri1, S D'Angeli1
1Istituto Sperimentale per la Frutticoltura, Ciampino Aeroporto I-00040 Rome, Italy e-mail: isf.propag@mclink.it Fax: +39-06-79340158, , , , , , IT.
Abstract:
A new reliable protocol for the induction of adventitious shoot formation and plant regeneration from apple callus has been developed. High regeneration frequency was obtained with this method in four different genotypes (Jork9, M26, Gala and McIntosh) and callus maintained regeneration ability for several months. The procedure consists of inducing vegetative shoot apices, excised from in vitro shoots, for 20 days in darkness on an MS medium without glycine, supplied with 17.8 μM BA, 2.7 μM NAA and 250 mg l-1 cefotaxime. The explants are then transferred to a fresh auxin-free medium and given light. Histological studies revealed that all the regenerated shoots originated from callus. Regenerated shoots were multiplied, rooted and successfully established in soil.
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