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Updated: Jan 29, 2026

A Method for Characterizing Embryogenesis in Arabidopsis
Published on: August 4, 2017
Efficient plant regeneration of Mesembryanthemum crystallinum via somatic embryogenesis
J C Cushman1, T Wulan1, N Kuscuoglu2
1Department of Biochemistry and Molecular Biology, 147 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma, USA Fax: +1-405-744-7799 e-mail: jcushman@biochem.okstate.edu, , , , , , US.
Abstract:
An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 μM 2,4-D and 1 μM kinetin. High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored abnormal embryo proliferation. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and morphologically identical to seed-derived plants.
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