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Updated: Jan 29, 2026
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High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
Published on: March 12, 2012
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Adventitious shoot formation induced by the root-knot nematode Meloidogyne hapla
1Department of Entomology and Nematology, IACR-Rothamsted, Harpenden, AL5 2JQ, UK e-mail: nicola.vonmende@bbsrc.ac.uk Fax: +44-1582-760981, , , , , , GB.
Plant Cell Reports
|February 14, 2019
Summary
Meloidogyne hapla infection in Arabidopsis thaliana roots causes extensive root production and occasional adventitious shoot formation. This contrasts with Meloidogyne incognita, which elicits minimal root growth and no shoot development.
Area of Science:
- Plant pathology
- Nematology
- Plant-microbe interactions
Background:
- Root-knot nematodes (Meloidogyne spp.) are significant agricultural pests.
- Understanding nematode-plant interactions is crucial for developing resistant crop varieties.
- Meloidogyne hapla and Meloidogyne incognita are distinct species with varying host interactions.
Purpose of the Study:
- To investigate the root infection process of Meloidogyne hapla in Arabidopsis thaliana.
- To compare the gall formation and developmental responses induced by M. hapla and M. incognita.
Main Methods:
- Monoxenic culture of Arabidopsis thaliana (L.) Heynh cv. Landsberg erecta.
- Infection assays with infective second-stage juveniles of Meloidogyne hapla.
- Microscopic observation of nematode migratory paths and gall development.
- Comparative analysis with Meloidogyne incognita infection.
Main Results:
- Meloidogyne hapla juveniles migrated towards and turned within the root tip to invade the vascular system.
- Extensive root production was observed at M. hapla feeding sites approximately one week post-invasion.
- Adventitious shoots formed in about 4.5% of galls induced by M. hapla.
- Minimal root production and no shoot formation were observed with M. incognita.
Conclusions:
- Meloidogyne hapla elicits distinct developmental responses in Arabidopsis thaliana roots, including gall formation, root proliferation, and adventitious shoot organogenesis.
- The observed responses differ significantly from those induced by Meloidogyne incognita, highlighting species-specific interactions.
- Arabidopsis thaliana serves as a model system for studying the molecular mechanisms underlying nematode-induced gall and shoot formation.
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