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Published on: November 23, 2016
An improved optical technique for monitoring plant cell concentration
1Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USA e-mail: jmlee@mail.wsu.edu Fax: +1-509-335-4806, , , , , , US.
Abstract:
An improved optical method was developed to determine cell weight concentrations using a micro-plate reader. Light absorbance was measured by a vertical light beam, which can minimize the cell settling effect found in traditional optical measurements with a horizontal light beam. The use of well plates not only requires very small sample sizes, but also handles a large number of samples at the same time. Absorbance measurements were linearly related to cell weight over the full range of batch culture growth.
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