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Re: "Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community" (2018) 6:87,

M S Payne1, J A Keelan2,3, L F Stinson2

  • 1Division of Obstetrics and Gynaecology, School of Medicine, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, Australia. matthew.payne@uwa.edu.au.

Microbiome
|February 14, 2019
PubMed
Summary

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This summary is machine-generated.

The microbiome in amniotic fluid is not solely from reagent contamination, contrary to a recent publication. Methodological concerns challenge the conclusion that the amniotic fluid microbiome is undetectable.

Area of Science:

  • Microbiology
  • Obstetrics
  • Genomics

Background:

  • Amniotic fluid was previously thought to be sterile.
  • A recent study suggested the amniotic fluid microbiome originates from reagent contamination.
  • This challenges the existence of an in-utero microbiome.

Discussion:

  • Re-examination of Lim et al. 2018 methods and supplementary data reveals inconsistencies.
  • The study's conclusions regarding reagent contamination are not fully supported by their own results.
  • Concerns regarding data interpretation and methodology require further discussion.

Key Insights:

  • The original study's conclusion that amniotic fluid lacks a microbiome is questionable.
  • Methodological flaws may have influenced the interpretation of results.
Keywords:
Amniotic fluidBacteriaMicrobiomePregnancyViruses

Related Experiment Videos

  • The potential existence of an in-utero microbiome warrants continued investigation.

    • Outlook:

    • Further research with refined methodologies is needed to accurately characterize the amniotic fluid microbiome.
    • Open discussion and collaboration are crucial for advancing our understanding of prenatal microbial colonization.
    • Clarifying the origin of the amniotic fluid microbiome is essential for prenatal and neonatal health research.