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Related Experiment Video

Updated: Jan 29, 2026

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
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Dual β-Lactam Combinations Highly Active against Mycobacterium abscessus Complex In Vitro.

R Pandey1, L Chen1, C Manca1

  • 1Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.

Mbio
|February 14, 2019
PubMed
Summary

Dual β-lactams, ceftazidime combined with ceftaroline or imipenem, show synergistic activity against Mycobacterium abscessus complex (MABC) infections. This finding offers hope for new treatment strategies for these difficult-to-treat chronic infections.

Keywords:
Mycobacterium abscessusbeta-lactamasesbeta-lactamsmultidrug resistance

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Area of Science:

  • Infectious Diseases
  • Microbiology
  • Pharmacology

Background:

  • Chronic infections caused by Mycobacterium abscessus complex (MABC) are increasing in immunocompromised individuals.
  • Macrolide resistance in MABC complicates treatment, necessitating novel therapeutic approaches.
  • Repurposing existing antibiotics offers a rapid strategy for developing new treatments.

Purpose of the Study:

  • To investigate the synergistic activity of dual β-lactam antibiotic combinations against MABC.
  • To evaluate the clinical relevance of these combinations in vitro and in infected human macrophages.

Main Methods:

  • Minimum Inhibitory Concentration (MIC) testing of β-lactam combinations against clinical MABC isolates.
  • Time-kill kinetic studies using clinically achievable concentrations of antibiotics.
  • Assessment of treatment efficacy in THP-1 human macrophages infected with MABC.

Main Results:

  • Ceftazidime combined with ceftaroline or imipenem demonstrated synergistic activity with low MIC50 values.
  • Dual β-lactam therapy achieved persistent bactericidal effects in time-kill studies.
  • Combination treatment significantly reduced MABC bacterial load in infected human macrophages.

Conclusions:

  • Dual β-lactam combinations, particularly ceftazidime with ceftaroline or imipenem, exhibit synergistic and clinically relevant activity against MABC.
  • These findings support the development of dual β-lactam regimens for treating MABC infections.
  • The study opens new avenues for repurposing β-lactam antibiotics against challenging MABC infections.