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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
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Sirt1 antisense transcript is down-regulated in human tumors.

Neda Mokhberian1, Seyed Mahmoud Hashemi2,3, Vahid Jajarmi4

  • 1Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Molecular Biology Reports
|February 14, 2019
PubMed
Summary

Natural antisense transcripts (NATs) regulate Sirt1 gene expression in cancer. Sirt1 mRNA increases while its antisense transcript (AS) decreases in tumors, suggesting a functional role for Sirt1-AS in cancer development.

Keywords:
Cancer cell lineHuman tumorNatural antisense transcriptSirt1Stem cell

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Regulation

Background:

  • Natural antisense transcripts (NATs) are implicated in human cancer development.
  • The Sirt1 gene, encoding a NAD-dependent deacetylase, shows altered mRNA expression during cancer progression.

Purpose of the Study:

  • To investigate the relationship between Sirt1 mRNA and its antisense transcript (Sirt1-AS) in various cancer models.
  • To determine the potential regulatory role of Sirt1-AS in Sirt1 mRNA expression.

Main Methods:

  • Quantitative RNA measurement was employed.
  • Expression levels of Sirt1 mRNA and Sirt1-AS were analyzed across human cancer tissues, cell lines, and stem cells.

Main Results:

  • Sirt1 mRNA levels were elevated in cancer cell lines and tissues compared to controls.
  • Conversely, Sirt1-AS expression was reduced in cancers.
  • An inverse correlation was observed between Sirt1 sense and antisense transcript levels in normal and cancerous tissues.

Conclusions:

  • Sirt1-AS exhibits an inverse correlation with Sirt1 mRNA in human cancers.
  • These findings suggest a functional role for Sirt1-AS in regulating Sirt1 mRNA expression within the context of cancer.