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A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
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Sirt1 antisense transcript is down-regulated in human tumors

Neda Mokhberian1, Seyed Mahmoud Hashemi2,3, Vahid Jajarmi4

  • 1Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Molecular Biology Reports
|February 14, 2019
PubMed

Insights

Natural antisense transcripts (NATs) regulate Sirt1 gene expression in cancer. Sirt1 mRNA increases while its antisense transcript (AS) decreases in tumors, suggesting a functional role for Sirt1-AS in cancer development.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Regulation

Background:

  • Natural antisense transcripts (NATs) are implicated in human cancer development.
  • The Sirt1 gene, encoding a NAD-dependent deacetylase, shows altered mRNA expression during cancer progression.

Purpose of the Study:

  • To investigate the relationship between Sirt1 mRNA and its antisense transcript (Sirt1-AS) in various cancer models.
  • To determine the potential regulatory role of Sirt1-AS in Sirt1 mRNA expression.

Main Methods:

  • Quantitative RNA measurement was employed.
  • Expression levels of Sirt1 mRNA and Sirt1-AS were analyzed across human cancer tissues, cell lines, and stem cells.

Main Results:

  • Sirt1 mRNA levels were elevated in cancer cell lines and tissues compared to controls.
  • Conversely, Sirt1-AS expression was reduced in cancers.
  • An inverse correlation was observed between Sirt1 sense and antisense transcript levels in normal and cancerous tissues.

Conclusions:

  • Sirt1-AS exhibits an inverse correlation with Sirt1 mRNA in human cancers.
  • These findings suggest a functional role for Sirt1-AS in regulating Sirt1 mRNA expression within the context of cancer.
Keywords:
Cancer cell lineHuman tumorNatural antisense transcriptSirt1Stem cell

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