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Gene expression variability in human skeletal muscle transcriptome responses to acute resistance exercise
Jacob T Bonafiglia1, Keir J Menzies2,3,4, Brendon J Gurd1
1School of Kinesiology and Health Studies, Queen's University, Kingston, ON, Canada.
Experimental Physiology
|February 14, 2019
Summary
Acute resistance exercise (ARE) influences gene expression variability. A reanalysis of microarray data shows ARE contributes to differences in gene expression responses, highlighting individual responsiveness in skeletal muscle adaptation.
Area of Science:
- Exercise Physiology
- Molecular Biology
- Genomics
Background:
- Individual responses to exercise vary significantly.
- Understanding gene expression changes after exercise is crucial for adaptation research.
Purpose of the Study:
- To determine if acute resistance exercise (ARE) itself causes variability in gene expression.
- To quantify the contribution of ARE to gene expression response variability.
Main Methods:
- Reanalysis of publicly available microarray data from skeletal muscle biopsies.
- Comparison of gene expression before and after ARE versus a control period.
- Statistical analysis to isolate ARE's effect on gene expression variability.
Main Results:
- Acute resistance exercise (ARE) contributed to gene expression variability in approximately 31,000 transcripts.
- A subset of 1290 genes showed significant variability attributable solely to ARE.
- These genes are involved in pathways regulating skeletal muscle adaptation.
Conclusions:
- Variability in gene expression responses to ARE is partly due to the exercise stimulus itself.
- Individual responsiveness plays a role in observed gene expression changes post-exercise.
- Findings suggest ARE influences specific molecular pathways relevant to muscle adaptation.
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