Related Experiment Video

Updated: Jan 29, 2026

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.6K

Phototriggered Release of a Transmembrane Chloride Carrier from an o-Nitrobenzyl-Linked Procarrier

Swati Bansi Salunke1, Javid Ahmad Malla1, Pinaki Talukdar1

  • 1Chemistry Department, Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune, 411008, Maharashtra, India.

Angewandte Chemie (International Ed. in English)
|February 14, 2019
PubMed

Insights

Researchers developed a light-activated chloride procarrier that releases an active carrier upon photoirradiation. This system efficiently targets and kills cancer cells, offering a potential new photodynamic therapy for cancer treatment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Photochemistry

Background:

  • Synthetic chloride carriers show potential for cancer therapy but face challenges with cytotoxicity to healthy cells.
  • Existing systems for targeting cancer have not fully realized their potential due to off-target effects.

Purpose of the Study:

  • To develop a novel photocleavable procarrier for targeted cancer therapy.
  • To investigate the efficiency of light-triggered chloride transport and cancer cell killing.

Main Methods:

  • Development of an indole-2-carboxamide receptor as a chloride carrier.
  • Synthesis of an o-nitrobenzyl-linked derivative as a photocleavable procarrier.
  • Photoirradiation experiments in liposomes and cancer cells to assess carrier release and transport efficiency.

Main Results:

  • The o-nitrobenzyl-linked derivative functions as an efficient procarrier, releasing the active chloride carrier upon photoirradiation.
  • Up to 90% transport efficiency was achieved in liposomes.
  • Photoreleased carrier demonstrated efficient cancer cell killing within cells.

Conclusions:

  • Photocleavable procarriers offer a promising strategy for targeted cancer therapy.
  • This system has potential as a novel photodynamic therapy to combat various cancers with reduced cytotoxicity.
Keywords:
anion bindingapoptosischlorideion carriersphotoactivation

More Related Videos

Designing Porous Silicon Films as Carriers of Nerve Growth Factor10:12

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Published on: January 25, 2019

10.2K
Dynamic Electrochemical Measurement of Chloride Ions07:32

Dynamic Electrochemical Measurement of Chloride Ions

Published on: February 5, 2016

12.0K

Related Concept Videos

Electron Carriers01:24

Electron Carriers

Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
91.7K
Energy-releasing Steps of Glycolysis01:28

Energy-releasing Steps of Glycolysis

Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
146.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.5K
Carrier Transport01:21

Carrier Transport

The generation of electrical current in semiconductors is fundamentally driven by two mechanisms: drift and diffusion. These processes are essential for the functionality and performance of semiconductor-based devices.
Drift Current:
The drift of charge carriers is started by an external electric field (E). Charged particles, such as electrons and holes, experience an acceleration between collisions with lattice atoms. For electrons, this results in a drift velocity (vd) given by:
946
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
6.6K
The ADP/ATP Carrier Protein01:42

The ADP/ATP Carrier Protein

ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
4.3K