Facile Synthesis of Cu₂O@TiO₂-PtCu Nanocomposites as a Signal Amplification Strategy for the Insulin Detection

Novel ultrasensitive sandwich-type electrochemical immunosensor was proposed for the quantitative detection of insulin, a representative biomarker for diabetes. To this end, molybdenum disulfide nanosheet-loaded gold nanoparticles (MoS₂/Au NPs) were used as substrates to modify bare glassy carbon electrodes. MoS₂/Au NPs not only present superior biocompatible and large specific surface area to enhance the loading capacity of primary antibody (Ab₁) but also present good electrical conductivity to accelerate electron transfer rate. Moreover, the amino-functionalized cuprous oxide decorated with titanium dioxide octahedral composites (Cu₂O@TiO₂-NH₂) were prepared to load dendritic platinum-copper nanoparticles (PtCu NPs) to realize signal amplification strategy. The resultant nanocomposites (cuprous oxide decorated with titanium dioxide octahedral loaded dendritic platinum-copper nanoparticles) demonstrate uniform octahedral morphology and size, which effectively increases the catalytically active sites and specific surface area to load the secondary antibody (Ab₂), even increases conductivity. Most importantly, the resultant nanocomposites possess superior electrocatalytic activity for hydrogen peroxide (H₂O₂) reduction, which present the signal amplification strategy. Under the optimal conditions, the proposed immunosensor exhibited a linear relationship between logarithm of insulin antigen concentration and amperometric response within a broad range from 0.1 pg/mL to 100 ng/mL and a limit detection of 0.024 pg/mL. Meanwhile, the immunosensor was employed to detect insulin in human serum with satisfactory results. Furthermore, it also presents good reproducibility, selectivity, and stability, which exhibits broad application prospects in biometric analysis.