Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Deconvolution01:20

Deconvolution

582
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
582
Mixtures of Acids03:27

Mixtures of Acids

21.7K
The pH of a solution containing an acid can be determined using its acid dissociation constant and its initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending upon the relative strength of the acids and their dissociation constants.
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
21.7K
Mixtures of Acids01:19

Mixtures of Acids

1.1K
The pH of a solution containing an acid can be determined using its acid dissociation constant and initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending on the relative strength of the acids and their dissociation constants.
In a strong and weak acid mixture, the strong acid dissociates completely and becomes a source of almost all the hydronium ions present in the solution. In contrast, the weak acid shows...
1.1K
Parallel Resonance01:23

Parallel Resonance

556
The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
556
Parallel Processing01:20

Parallel Processing

708
The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
708
Resistors In Parallel01:23

Resistors In Parallel

6.1K
Resistors are in parallel when one end of all the resistors are connected to a continuous wire of negligible resistance and the other end of all the resistors are also connected to one another through a continuous wire of negligible resistance. In the case of a parallel configuration, the potential drop across each resistor is the same. Current through each resistor can be found using Ohm’s law, I = V/R, where the voltage is constant across each resistor. The sum of the individual currents...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain-gut axis imaging, motion correction with [ <sup>11</sup> C]-carfentanil total-body PET.

medRxiv : the preprint server for health sciences·2026
Same author

The Clinical and Surgical Landscape of Gender Affirming Vocal Care: A Scoping Review.

Laryngoscope investigative otolaryngology·2026
Same author

Optimization of DNA collection card workflows on the RapidHIT™ ID instrument.

International journal of legal medicine·2026
Same author

Evaluating Components of Vocal Effort in Transgender Women.

Laryngoscope investigative otolaryngology·2026
Same author

Eosinophils in the immunologic spectrum of Eosinophilic Granulomatosis with Polyangiitis: From Fundamental Pathobiology to Emerging Therapeutic Strategies and Future Perspectives.

Internal medicine (Tokyo, Japan)·2026
Same author

Evaluating GPT-4o for Automated Classification of Skin Lesions Using the HAM10000 Dataset.

Cutis·2026

Related Experiment Video

Updated: Jan 29, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.9K

Mixture deconvolution by massively parallel sequencing of microhaplotypes.

Lindsay Bennett1, Fabio Oldoni2, Kelly Long2

  • 1Metro Nashville Police Department Crime Laboratory, 400 Myatt Drive, Madison, TN, 37115, USA.

International Journal of Legal Medicine
|February 14, 2019
PubMed
Summary
This summary is machine-generated.

Massively parallel sequencing of microhaplotypes offers enhanced forensic human identification, particularly for complex DNA mixtures. This method overcomes limitations of short tandem repeat polymorphisms (STRs) by avoiding stutter fragments and improving minor contributor detection.

Keywords:
Forensic DNA samplesMassively parallel sequencing (MPS)MicrohaplotypeMixture deconvolutionSingle-nucleotide polymorphism

More Related Videos

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.7K
Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.4K

Related Experiment Videos

Last Updated: Jan 29, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.9K
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.7K
Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.4K

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Short tandem repeat polymorphisms (STRs) are standard for human identification but have limitations with complex mixtures, including stutter fragments and allele drop-out.
  • Massively parallel sequencing (MPS) offers higher discrimination and access to new markers like microhaplotypes (MHs).
  • Microhaplotypes are short, highly polymorphic loci with alleles of identical length, producing no stutter fragments, making them promising for mixture analysis.

Purpose of the Study:

  • To practically demonstrate the utility of microhaplotypes for forensic mixture interpretation.
  • To evaluate a panel of 36 microhaplotypes using MPS for human identification.
  • To compare the performance of MPS with microhaplotypes against traditional STR analysis in complex mixtures.

Main Methods:

  • A panel of 36 microhaplotypes was tested on the Ion S5™ MPS platform.
  • Samples included single-source DNA, synthetic mixtures (2-6 contributors), and casework-like samples.
  • The microhaplotype panel was analyzed both multiplexed with STRs/SNPs and individually.

Main Results:

  • Single-source sample analysis showed good allele coverage ratios, comparable to STRs analyzed by capillary electrophoresis (CE).
  • Mixture studies demonstrated higher input DNA tolerance and increased sensitivity for minor contributors in imbalanced mixtures due to the absence of stutter fragments.
  • MPS of microhaplotypes detected more minor donor alleles in casework-like samples compared to MPS and CE of STRs.

Conclusions:

  • Massively parallel sequencing of microhaplotypes effectively complements STR analysis in forensic human identification.
  • Microhaplotypes significantly enhance the interpretation of complex and imbalanced DNA mixtures.
  • This approach offers improved sensitivity and reliability for forensic casework.