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Related Experiment Video

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Pyrosequencing for Microbial Identification and Characterization
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Engineering Nanowire-Mediated Cell Lysis for Microbial Cell Identification.

Takao Yasui1, Takeshi Yanagida2,3, Taisuke Shimada

  • 1Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO) , 4-1-8 Honcho , Kawaguchi , Saitama 332-0012 , Japan.

ACS Nano
|February 14, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel nanowire-mediated lysis method for robust microbial cells. This technique uses membrane stretching for efficient cell disruption, enabling rapid identification assays.

Keywords:
intracellular analysisloop-mediated isothermal amplificationmicrobial cellmicrofluidicsnanowire-mediated lysisnanowires

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Microbiology

Background:

  • Inorganic nanowires show promise for cell analysis.
  • Current microbial cell lysis methods are insufficient due to cell robustness.
  • Nanowire penetration is not an effective lysis strategy for microbial cells.

Purpose of the Study:

  • To develop an effective nanowire-mediated lysis method for microbial cells.
  • To investigate the mechanism of nanowire-mediated lysis through cell membrane stretching.
  • To integrate nanowire lysis with loop-mediated isothermal amplification for rapid identification.

Main Methods:

  • Proposed a nanowire-mediated lysis method utilizing cell membrane stretching and shear force.
  • Demonstrated lysis of Gram-positive bacteria, Gram-negative bacteria, and yeast cells.
  • Integrated nanowire lysis with loop-mediated isothermal amplification (LAMP) for a one-step assay.

Main Results:

  • The nanowire-mediated lysis effectively disrupts microbial cell membranes via stretching, not penetration.
  • The method is compatible with Gram-positive bacteria, Gram-negative bacteria, and yeast.
  • Integration with LAMP resulted in a simple, rapid, one-step visual chromatic identification assay.

Conclusions:

  • Nanowire-mediated lysis offers a new approach for microbial cell disruption.
  • This method enables rapid and simple microbial identification assays, compatible with LAMP.
  • The developed platform facilitates on-site microbial detection without contamination, including resistant strains.