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Related Experiment Video

Updated: Jan 29, 2026

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Published on: September 20, 2024

Leaf Whorl Inoculation Method for Screening Sugarcane Rust Resistance.

Sushma G Sood¹, Jack C Comstock¹, Neil C Glynn¹

¹United States Department of Agriculture-Agricultural Research Service, Sugarcane Field Station, Canal Point, FL 33438.

|February 15, 2019

Summary

This summary is machine-generated.

A new leaf whorl inoculation technique effectively screens sugarcane for resistance to brown and orange rust diseases. This method provides a reliable way to identify resistant sugarcane cultivars, improving disease management strategies.

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Area of Science:

Plant Pathology
Agronomy
Crop Science

Background:

Brown rust (Puccinia melanocephala) and orange rust (P. kuehnii) are significant sugarcane diseases in Florida.
Cultivar resistance is the primary control method, but natural infection for screening is unreliable due to environmental variability.

Purpose of the Study:

To evaluate a novel inoculation technique for identifying sugarcane resistance to brown and orange rust.
To establish optimal inoculum concentrations for reliable disease symptom development.

Main Methods:

Field inoculations using a urediniospore suspension (0.5 ml) applied to the leaf whorl of sugarcane stalks.
Determining optimal inoculum concentrations: 10^5 urediniospores/ml for brown rust and 10^4 urediniospores/ml for orange rust.
Screening sugarcane clones from the Canal Point breeding program using the leaf whorl inoculation method.

Main Results:

Symptoms appeared on susceptible cultivars within 4 weeks post-inoculation.
The leaf whorl inoculation technique allowed for rapid screening of numerous cultivars.
The method requires minimal inoculum and labor, proving efficient for field plantings.

Conclusions:

The leaf whorl inoculation technique is a reliable and efficient method for assessing sugarcane resistance to brown and orange rust.
This technique facilitates the selection of resistant sugarcane genotypes, crucial for disease management.