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Immunomodulation of ABA function affects early events in somatic embryo development.

S Senger1, H-P Mock1, U Conrad1

  • 1Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: manteuffel@ipk-gatersleben.de Fax: +49-39482 5139, , , , , , DE.

Plant Cell Reports
|February 15, 2019
PubMed
Summary

Abscisic acid (ABA) is crucial for early plant embryo development. Blocking ABA function or deficiency disrupts somatic embryogenesis in Nicotiana plumbaginifolia, but restoring ABA levels normalizes development.

Keywords:
ABA biosynthesis mutantsAnti-ABA single chain fragment variableKeywords Abscisic acidNicotiana plumbaginifoliaSomatic embryogenesis

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Area of Science:

  • Plant Biotechnology
  • Plant Physiology
  • Developmental Biology

Background:

  • Somatic embryogenesis is a key process in plant biotechnology for asexual propagation.
  • The role of phytohormones, particularly abscisic acid (ABA), in early embryonic events is not fully understood.

Purpose of the Study:

  • To investigate the role of abscisic acid (ABA) in the early stages of somatic embryogenesis in Nicotiana plumbaginifolia.
  • To demonstrate the direct effect of ABA on crucial early embryonic events using immunomodulation.

Main Methods:

  • Generation of a transgenic Nicotiana plumbaginifolia line expressing an anti-ABA antibody in the endoplasmic reticulum.
  • Comparative analysis of somatic embryo development between transgenic and wild-type lines.
  • Utilizing ABA biosynthesis mutants (aba1, aba2) and fluridone treatment to create ABA-deficient conditions.

Main Results:

  • Disruption of ABA function or ABA deficiency led to disturbed development of embryonic structures in early stages.
  • Normal somatic embryo development was restored upon ABA complementation in in vitro cultures.
  • This study provides the first evidence for ABA's direct impact on early embryonic events during somatic embryogenesis.

Conclusions:

  • Abscisic acid (ABA) plays a critical and indispensable role in the early developmental stages of somatic embryogenesis.
  • Modulating ABA levels or function offers a potential strategy for controlling plant somatic embryo development.