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Published on: June 9, 2017
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Cunxi Wang · Kevan P.C. Croft · David F. Hildebrand
C Wang1, K P C Croft1, D F Hildebrand1
1Department of Agronomy, University of Kentucky, Lexington, KY 40546, USA e-mail: dhild@pop.uky.edu Fax: +1-606-2577874, , , , , , US.
Plant Cell Reports
|February 15, 2019
Summary
Auxin (α-naphthaleneacetic acid) induces specific lipoxygenases (LOXs) in soybean embryos, identified as seedling LOX4. This suggests auxin plays a role in LOX gene expression during seed germination.
Area of Science:
- Plant Biochemistry
- Molecular Biology
- Soybean Genetics
Background:
- Lipoxygenases (LOXs) are enzymes involved in plant development and defense.
- Auxins, like α-naphthaleneacetic acid (NAA), are plant hormones regulating growth and gene expression.
- Soybean (Glycine max) embryo cotyledons express specific LOX isoforms.
Purpose of the Study:
- To investigate the effect of auxin on lipoxygenase expression in soybean embryo cotyledons.
- To characterize the auxin-induced LOXs and compare them to known seedling LOXs.
- To determine if auxin influences the expression of specific LOX genes (LOX4 and LOX5).
Main Methods:
- Culture of immature zygotic embryo cotyledons.
- Induction of LOX expression using auxin (NAA).
- Isoelectric focusing (pI) and molecular mass determination of induced LOXs.
- Partial amino acid sequencing of purified LOX.
- RNA protection assays to quantify mRNA levels.
- Analysis of soybean genotypes with polymorphic LOX4 variants.
Main Results:
- NAA induced LOX expression in soybean embryo cotyledons, distinct from seed-expressed LOXs.
- One auxin-induced LOX shared pI (5.09) and molecular mass (94 kDa) with seedling LOX4.
- Partial amino acid sequences confirmed the identity of the NAA-induced LOX as LOX4.
- NAA upregulated LOX4 and LOX5 mRNA in cultured embryo cotyledons.
- Soybean genotypes exhibited consistent LOX4 variation in both hypocotyls and NAA-induced embryo cotyledons.
Conclusions:
- The auxin-induced pI-5.09 LOX in soybean embryo cotyledons is identified as seedling LOX4.
- Auxin may directly or indirectly regulate seedling LOX gene expression during soybean seed germination.
- This finding provides insights into the hormonal control of gene expression in plant development.
