Related Experiment Video

Updated: Jan 29, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.3K

Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma

Chao Shang1, Cheng N Ao2, Chi C Cheong2

  • 1Department of Neurobiology, School of Life Science, China Medical University, Shenyang, China.

Frontiers in Oncology
|February 15, 2019
PubMed

Insights

High expression of CDKN2B antisense RNA 1 (CDKN2B-AS) in endometrial cancer (EC) correlates with poor paclitaxel sensitivity. Knocking down CDKN2B-AS enhances chemotherapy response by targeting the miR-125a-5p/Bcl2/MRP4 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Endometrial cancer (EC) is the most common gynecologic malignancy.
  • Paclitaxel is a key chemotherapy agent for EC treatment.
  • Understanding chemoresistance mechanisms is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the clinical significance of CDKN2B antisense RNA 1 (CDKN2B-AS) in EC.
  • To elucidate the role of CDKN2B-AS in paclitaxel sensitivity and chemoresistance.
  • To explore the underlying molecular pathways involving miR-125a-5p, Bcl2, and MRP4.

Main Methods:

  • Gene expression analysis in EC tissues and cell lines.
  • In vitro experiments involving gene knockdown and upregulation.
  • Assessment of cell sensitivity to paclitaxel and cytotoxicity.
  • Analysis of gene and microRNA interactions (e.g., targeting relationships).

Main Results:

  • CDKN2B-AS was highly expressed in EC tissues and associated with poor paclitaxel sensitivity.
  • Knockdown of CDKN2B-AS resensitized EC cells to paclitaxel and increased cytotoxicity.
  • Low miR-125a-5p expression correlated with paclitaxel resistance; its upregulation enhanced sensitivity.
  • CDKN2B-AS knockdown's effect was mediated by miR-125a-5p, which targets Bcl2 and MRP4.

Conclusions:

  • High CDKN2B-AS expression indicates a poor response to paclitaxel in EC patients.
  • CDKN2B-AS knockdown inhibits paclitaxel resistance via the miR-125a-5p-Bcl2/MRP4 pathway.
  • This study elucidates molecular mechanisms of chemoresistance in EC, offering potential therapeutic targets.
Keywords:
CDKN2B antisense RNA 1chemotherapyendometrial carcinomamiR-125a-5pnon-coding RNApaclitaxel

More Related Videos

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.1K
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

7.8K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.6K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.8K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.0K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.1K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.0K