Related Experiment Video
Updated: Jan 29, 2026

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Published on: June 28, 2018
Screening data for the endocrine disrupting activities of 583 chemicals using the yeast two-hybrid assay
Fujio Shiraishi1, Ryo Kamata2, Masanori Terasaki3
1National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
Abstract:
We screened 583 chemicals for receptor binding activity to the human estrogen receptor (hER), the Japanese medaka estrogen receptor (medER), and the aryl hydrocarbon receptor (AhR) using the yeast two-hybrid assay. The substances tested included substances that could potentially be produced unintentionally by industrial processes, such as halogenated steroids and phenols. Antagonistic effects on hER and the androgen receptor were also screened. The test chemicals were selected for screening on the basis of chemical structure associated with possible estrogen receptor binding activity. The current study presents the report on the screening of 583 chemicals for different kinds of endocrine disrupting activity.
Related Concept Videos
Chemical Signaling in the Endocrine System
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
Endocrine Signaling
Yeast Signaling
What is the Endocrine System?
The Endocrine System
Thermodynamics: Chemical Potential and Activity
The thermodynamic equilibrium constant is more accurately defined in terms of activity rather than concentration.

