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Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
Published on: January 19, 2012
Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos
Christina Murko1,2, Felipe Monteleone Vieceli1, Marianne Bronner1
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Abstract:
We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.
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