Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

33.7K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.7K
GTPases and their Regulation02:14

GTPases and their Regulation

9.8K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.8K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Positive Regulator Molecules01:45

Positive Regulator Molecules

136.1K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Returning to Work After Maternity Leave: A Qualitative Study of Nurses' Experiences and Factors Influencing Their Retention.

Nursing open·2026
Same author

Changes in the Proteome and Phosphoproteome of <i>Zea mays</i> Tissues in Drought Stress Show Plant Tissue Responses from Dehydrins, Carboxylic Acid Metabolism, RNA Splicing and Transcription Factors.

Proteomes·2026
Same author

Adolescent Adapted Wheelchair Sport Athlete Baseline Evaluation of Concussion-Like Symptoms.

Journal of athletic training·2026
Same author

The political polarization of health outcomes in the USA.

Nature human behaviour·2026
Same author

Inhibition of cytomegalovirus reactivation by ex vivo treatment of human kidneys with the SYN002 immunotoxin.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Expression of Concern: Inhibiting EGF Receptor or SRC Family Kinase Signaling Overcomes BRAF Inhibitor Resistance in Melanoma.

Cancer discovery·2026
Same journal

Candida albicans hyphae modulate Staphylococcus aureus cell-free supernatant during dual biofilm growth to drive molecular signatures of oral dysplasia.

Medical microbiology and immunology·2026
Same journal

Targeting phospholipase A2 with arachidonyl trifluoromethyl ketone modulates macrophage activation during Japanese encephalitis virus infection.

Medical microbiology and immunology·2026
Same journal

Epitope mapping and conservation of antigens from the Multi-Cruzi immunoassay platform.

Medical microbiology and immunology·2026
Same journal

Unique molecular profiling of monocyte responses to a high dose of DENV-NS1 reflected the effect of NS1 on hemostasis.

Medical microbiology and immunology·2026
Same journal

Simultaneous quantification of anti-Spike IgG subclasses against various SARS-CoV-2 variants using a multiplex serological assay.

Medical microbiology and immunology·2026
Same journal

Intrauterine exposure to HBsAg attenuates the immune response to hepatitis B vaccine via the miR-155-5p/MyD88 axis of DCs in mouse pups.

Medical microbiology and immunology·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.8K

HCMV latency: what regulates the regulators?

Elizabeth Elder1, John Sinclair2

  • 1Department of Medicine, University of Cambridge, Box 157 Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ, UK. ege22@cam.ac.uk.

Medical Microbiology and Immunology
|February 15, 2019
PubMed
Summary
This summary is machine-generated.

Human cytomegalovirus (HCMV) latency relies on chromatin regulation at the MIEP. The viral protein US28 suppresses MIEP in myeloid cells, crucial for HCMV latency and a potential antiviral target.

Keywords:
Cell signallingChromatinCytomegalovirusLatencyUS28Viral reservoir

More Related Videos

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.9K
Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

807

Related Experiment Videos

Last Updated: Jan 29, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.8K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.9K
Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

807

Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Human cytomegalovirus (HCMV) latency and reactivation are controlled by chromatin structure at the major immediate early promoter (MIEP) in myeloid cells.
  • Both cellular and viral factors influence MIEP regulation during HCMV latency.

Purpose of the Study:

  • To review known mechanisms of MIEP regulation during HCMV latency.
  • To focus on the role of the viral G-protein coupled receptor, US28, in suppressing the MIEP.
  • To explore US28's function in maintaining HCMV latency and its potential as an antiviral target.

Main Methods:

  • Review of existing literature on MIEP regulation and US28 function.
  • Analysis of cellular signaling pathways modulated by US28.
  • Discussion of US28's role in CD34+ progenitor cells and CD14+ monocytes.

Main Results:

  • US28, a viral G-protein coupled receptor, actively suppresses the MIEP in early myeloid lineage cells.
  • US28 is essential for HCMV latency in CD34+ progenitor cells and CD14+ monocytes.
  • US28 modulates cellular signaling pathways to enforce MIEP suppression and regulate HCMV latency.

Conclusions:

  • US28 plays a critical role in establishing and maintaining HCMV latency by suppressing the MIEP.
  • US28's ability to 'regulate the regulators' highlights its significance in viral latency.
  • Cell-surface US28 represents a promising therapeutic target for eliminating the latent HCMV reservoir.