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Related Experiment Video

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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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NIR Biosensing of Neurotransmitters in Stem Cell-Derived Neural Interface Using Advanced Core-Shell Upconversion

Hudifah Rabie1, Yixiao Zhang1, Nicholas Pasquale1

  • 1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.

Advanced Materials (Deerfield Beach, Fla.)
|February 15, 2019
PubMed
Summary

Researchers developed advanced upconversion nanoparticles (UCNPs) for sensitive neurotransmitter detection. This breakthrough aids neurodegenerative disease research and regenerative medicine by enabling real-time monitoring of stem cell differentiation.

Keywords:
NIR biosensorscore-shell nanostructuresdetection of dopamineenergy migrationneurotransmittersstem cell differentiationupconversion nanoparticles

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Neuroscience

Background:

  • Nondestructive neurotransmitter detection and stem cell monitoring are crucial for neurodegenerative disease and regenerative medicine.
  • Existing luminescent biosensors face limitations like autofluorescence and phototoxicity.
  • Upconversion nanoparticles (UCNPs) offer advantages but suffer from low emission intensity.

Purpose of the Study:

  • To develop highly sensitive upconversion nanoparticles (UCNPs) with enhanced luminescence for biosensing.
  • To create a novel biosensor for real-time, in situ detection of neurotransmitters released from stem cell-derived neurons.
  • To investigate the role of neurotransmitters in neurological processes using advanced imaging techniques.

Main Methods:

  • Fabrication of a single-crystal core-shell-shell "sandwich" structured UCNP to minimize energy back-transfer and enhance luminescence.
  • Utilizing low power density excitation for bright visible emissions.
  • Development of a near-infrared (NIR)-based biosensor for dopamine detection in stem cell-derived neural interfaces.

Main Results:

  • The novel UCNPs exhibited significantly enhanced luminescent output compared to conventional UCNPs.
  • The developed biosensor demonstrated ultrasensitive detection of dopamine released from stem cell-derived dopaminergic-neurons.
  • The UCNP-based biosensor proved effective for in situ neurotransmitter detection in neural interfaces.

Conclusions:

  • The developed core-shell-shell UCNPs provide a superior platform for luminescent biosensing.
  • This advanced biosensing technology offers a unique tool for studying neurotransmitter roles in neurological processes at the single-cell level.
  • The findings hold promise for advancing research in neurodegenerative diseases and regenerative medicine.