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Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Related Experiment Video

Updated: Jan 29, 2026

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
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Network-Based Combinatorial CRISPR-Cas9 Screens Identify Synergistic Modules in Human Cells.

Yucheng Guo1, Chen Bao1, Dacheng Ma1

  • 1MOE Key Laboratory of Bioinformatics and TCM-X Center/Bioinformatics Division/TFIDT, BNRist, Department of Automation , Tsinghua University , Beijing 100084 , China.

ACS Synthetic Biology
|February 15, 2019
PubMed
Summary

This study introduces a network strategy to find gene modules driving inflammation-induced cancer. It identifies synergistic gene interactions in immune, metabolism, and proliferation pathways, offering potential targets for cancer prevention.

Keywords:
CRISPR-Cas9combinatorial screennetworksynergistic module

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Area of Science:

  • Oncology
  • Systems Biology
  • Genetics

Background:

  • Tumorigenesis involves complex gene networks and environmental factors.
  • Identifying functional gene networks perturbed during tumorigenesis remains challenging.

Purpose of the Study:

  • To develop a network-based strategy for discovering functional synergistic modules causal to inflammation-induced tumorigenesis.
  • To identify potential early combinational targets and biomarkers for inflammation-induced tumorigenesis.

Main Methods:

  • Integrated clinical and network-based genome-wide gene prediction for candidate genes.
  • Combinatorial CRISPR-Cas9 screening to identify functional synergistic modules.
  • Construction of an inflammation-induced differential genetic interaction network using a TGFβ1 model in colonic epithelial cells.

Main Results:

  • Identified opposing differential genetic interactions: synergistic promotion and suppression in tumorigenesis.
  • Synergistic promotion linked to immune and metabolism module deletions.
  • Synergistic suppression linked to proliferation/immune or proliferation/metabolism module deletions.

Conclusions:

  • The study highlights synergistic effects among immune, proliferation, and metabolism modules in inflammation-induced tumorigenesis.
  • Provides insights into mechanisms underlying inflammation-associated colon cancer risk.
  • Suggests novel functional modules and synergistic combinations for modulating complex disease phenotypes.