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Ligase-Controlled Cyclo-oligomerization of Peptides
Xinya Hemu1, Xiaohong Zhang1, James P Tam1
1School of Biological Sciences , Nanyang Technological University , 60 Nanyang Drive , Singapore 637551.
Abstract:
A biomimetic one-step ligase-catalyzed cyclo-oligomerization mediated by butelase 1, an Asn/Asp-specific ligase, is introduced that is time-, concentration-, length-, and sequence-dependent. This reaction yields cyclic mono-, di-, tri-, and tetramers from peptide precursors containing 3-15 amino acids ended with Asn and a His-Val tail. The cyclomonomers were favored when the peptide lengths were >9 amino acids. A turn-forming Pro residue at the P2 position favored the formation of higher-order cyclo-oligomers.
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