Related Experiment Video For Carbapenemase

Updated: Jan 29, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

Published on: September 16, 2013

Performance evaluation of the PANA RealTyper™ CRE Kit for detecting carbapenemase genes in Gram-negative bacilli

Jun Sung Hong¹, Dokyun Kim¹, Eun-Jeong Yoon¹

¹Department of Laboratory Medicine and Research Institute of Bacterial Resistance, College of Medicine, Yonsei University, Seoul, South Korea.

Journal of Global Antimicrobial Resistance

|February 15, 2019

Abstract

Objectives:

The spread of carbapenemase-producing organisms has been continuously reported over the past decade. Rapid and accurate detection of carbapenemase production is essential for adequate infection control and appropriate antimicrobial treatment in the clinical setting. In this study, the performance of the newly developed PANA RealTyper™ CRE Kit (PANAGENE, Daejeon, South Korea) for the detection of six common carbapenemase genes (bla_KPC, bla_GES, bla_IMP, bla_NDM, bla_VIM and bla_OXA-48-like) was evaluated.

Methods:

A total of 479 non-duplicate clinical isolates of Gram-negative bacilli, including 391 carbapenemase-producers and 88 non-producers, were tested. Conventional PCR and sequencing were performed as reference methods for performance evaluation of the PANA RealTyper™ CRE Kit.

Results:

The PANA RealTyper™ CRE Kit showed a reliable performance for the detection of carbapenemase-producing organisms compared with the monoplex PCR system, with sensitivity and specificity values both >95%, with only a few discrepant results for six types of carbapenemase genes.

Conclusion:

This kit is rapid and accurate for simultaneously detecting various carbapenemase genes in a single reaction and could contribute to early decisions for appropriate antimicrobial treatment in the clinical setting.

Keywords:

Carbapenemase Carbapenemase-producing organism Evaluation Gram-negative bacilli Real-time PCR

More Related Videos

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Positive, Negative, and Zero Work

Positive, Negative, and Zero Work

Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...

Negative and Cognitive Symptoms of Schizophrenia

Negative and Cognitive Symptoms of Schizophrenia

Negative symptoms of schizophrenia indicate a reduction or absence of typical behaviors and emotional responses found in healthy individuals, while positive symptoms reflect an excess or distortion of normal functioning.
Negative Symptoms
Negative symptoms of schizophrenia manifest as deficits in normal emotional and behavioral functioning, profoundly impacting daily life. Individuals with schizophrenia often display a flat affect, characterized by a near-total absence of emotional expression,...

Gene Families

Gene Families

Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...

Positive and Negative Feedback Loops

Positive and Negative Feedback Loops

Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires maintaining an internal dynamic equilibrium:

Keywords:

Carbapenemase Carbapenemase-producing organism Evaluation Gram-negative bacilli Real-time PCR

Articles linked to this work by shared authors, journal, and citation graph.

Persistent heavy metal contamination at the East Sea-Byeong dumping site: Tracing the impacts of ocean dumping and policy changes over two decades.

Environmental pollution (Barking, Essex : 1987)·2026

A Decade of Achievements and Future Directions in Global Antimicrobial Resistance Surveillance System in Korea (Kor-GLASS).

Public health weekly report·2026

Diverse Genomic Mechanisms of cfiA Activation in Carbapenem-Resistant Bacteroides fragilis Clinical Isolates from Korea.

Antibiotics (Basel, Switzerland)·2026

Development and Validation of Mock Communities as Quality Control Materials for Clinical Metagenomic Next-Generation Sequencing.

Annals of laboratory medicine·2026

Genomic analysis of carbapenem-resistant non-baumannii Acinetobacter harboring chromosomal bla OXA-23 or plasmid-mediated bla NDM-1, bla IMP-14, and bla OXA-58.

Frontiers in microbiology·2026

Changes in the Gut Microbiome Following Perioperative Prophylactic Cefazolin Administration in Patients Undergoing Orthopedic Surgery: A Longitudinal Prospective Study.

Antibiotics (Basel, Switzerland)·2026

See all related articles