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Current Coverage of the mTOR Pathway by Next-Generation Sequencing Oncology Panels

Rita Seeboeck1,2, Victoria Sarne3, Johannes Haybaeck4,5,6,7

  • 1Clinical Institute of Pathology, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, 3100 St. Pölten, Austria. rita.seeboeck@stpoelten.lknoe.at.

International Journal of Molecular Sciences
|February 16, 2019
PubMed

Insights

Next-generation sequencing (NGS) panels are increasingly covering mTOR pathway genes for cancer drug development. This review assesses NGS panel coverage of mTOR pathway genes, aiding diagnostic and research platform selection.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • The mechanistic target of rapamycin (mTOR) pathway is a critical target for novel oncological drugs and targeted therapies.
  • The inclusion of mTOR pathway genes in next-generation sequencing (NGS) oncology panels is rapidly expanding.
  • Understanding the coverage of these genes within diagnostic and research panels is crucial for precision medicine.

Purpose of the Study:

  • To review and analyze the coverage of the mTOR pathway within commercially available medium-sized diagnostic and research-oriented NGS panels.
  • To compare the mTOR pathway gene content of 16 DNA-based NGS panels and Foundation One.
  • To highlight emerging mTOR-associated somatic mutations not yet included in current panels, particularly eukaryotic translation initiation factors (eIFs).

Main Methods:

  • Systematic review of 16 medium-sized DNA-based NGS oncology panels.
  • Analysis of the gene list of Foundation One as a reference standard.
  • Identification and overview of mTOR-associated somatic mutations relevant to diagnostics and drug targeting.

Main Results:

  • The study provides a comprehensive summary of mTOR pathway coverage across various NGS panels.
  • Foundation One's gene list is used as a benchmark for comparison.
  • Emerging targets like eukaryotic translation initiation factors (eIFs) and their associated mutations are discussed.

Conclusions:

  • Awareness of the true coverage of NGS panels for the mTOR pathway is essential for informed selection.
  • This review aids researchers and clinicians in choosing the optimal NGS platform for oncological diagnostics and research.
  • The findings support the growing importance of the mTOR pathway and its downstream effectors in precision oncology.
Keywords:
IonTorrentNGSeIFsilluminamTOR

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