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Digital immunohistochemistry implementation, training and validation: experience and technical notes from a large
Bethany Jill Williams1,2, Dharshana Jayewardene2, Darren Treanor3,2
1Histopathology Department, Bexley Wing, St James University Hospital, Leeds, UK bethany.williams2@nhs.net.
Journal of Clinical Pathology
|February 16, 2019
Summary
Digital immunohistochemistry (IHC) offers value in clinical labs. A robust validation and implementation approach ensures accurate digital slide assessment, improving pathologist confidence and workflow efficiency.
Area of Science:
- Digital pathology
- Clinical laboratory services
- Immunohistochemistry
Background:
- Clinical immunohistochemistry (IHC) is crucial for diagnosis.
- Digitization offers potential benefits for IHC services.
- Implementation requires careful planning and validation.
Purpose of the Study:
- To evaluate the value of digitizing clinical IHC services.
- To develop an approach for digital IHC implementation and validation.
- To assess the feasibility in a large clinical laboratory setting.
Main Methods:
- Developed a slide scanning methodology.
- Implemented a novel validation exercise for pathologists.
- Assessed digital IHC slides for reporting strengths and weaknesses.
Main Results:
- 1480 digital IHC slides assessed by 24 pathologists.
- Complete clinical concordance between digital and glass slides.
- Rescanning at ×40 improved assessment confidence for difficult stains.
Conclusions:
- Well-managed digitization minimizes review delays.
- Careful validation of digital pathology systems and protocols is essential.
- Pathologists require training before transitioning to digital microscopy for routine IHC.
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