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Related Experiment Video

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Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition.

Samadrita Bhattacharyya1, Jialei Duan2, Lin Wang1

  • 1Department of Internal Medicine, Division of Cardiology, UT Southwestern Medical Center, Dallas, TX, 75390, USA.

Scientific Reports
|February 16, 2019
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Summary

Researchers developed a new Gjd3-CreEGFP mouse model for precise genetic manipulation of the atrioventricular node (AVN). This tool aids in studying AVN function and cellular makeup, offering a 3D view and single-cell atlas.

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Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Genetics and Genomics

Background:

  • The atrioventricular node (AVN) is crucial for coordinating heart contractions, optimizing cardiac output.
  • Studying AVN function in mice requires specific genetic tools for targeted manipulation.
  • Existing methods lack the specificity needed for detailed AVN research.

Purpose of the Study:

  • To develop a novel mouse model for AVN-specific genetic recombination.
  • To characterize the morphology and cellular composition of the AVN.
  • To establish a foundation for future research into AVN cellular heterogeneity and function.

Main Methods:

  • Generation of a Gjd3-CreEGFP reporter mouse line utilizing endogenous Gjd3 regulatory elements.
  • Histological analysis to confirm CreEGFP expression specificity in AVN cardiomyocytes and endothelial cells.
  • Assessment of cardiac function (mechanical and electrical) in the new mouse line.
  • Application of tissue clearing and confocal microscopy for 3D AVN visualization.
  • Microdissection guided by the Gjd3-CreEGFP model for single-cell atlas construction.

Main Results:

  • The Gjd3-CreEGFP mouse line enables reliable, AVN-specific recombination starting at embryonic day 12.5.
  • Histology confirmed specific labeling of AVN cardiomyocytes and a subset of cardiac endothelial cells.
  • The Gjd3-CreEGFP mice exhibit normal cardiac mechanical and electrical function.
  • A detailed 3D model of the AVN was generated, revealing distinct substructures.
  • The study successfully created a single-cell atlas of the AVN, paving the way for further investigation.

Conclusions:

  • The novel Gjd3-CreEGFP mouse line is a valuable tool for AVN-specific genetic studies.
  • The research provides an updated, high-resolution 3D morphological model of the AVN.
  • This work establishes a framework for dissecting AVN cellular diversity and function.