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Real-time imaging of senescence in tumors with DNA damage

Ying Wang1,2, Jun Liu1,2,3, Xiaowei Ma1,2,3

  • 1Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131, USA.

Scientific Reports
|February 16, 2019
PubMed

Insights

Researchers developed a novel near-infrared (NIR) molecular probe for detecting cellular senescence. This probe, activated by senescence-associated β-galactosidase (SABG), enables effective NIR imaging of senescent cells in vitro and in vivo.

Area of Science:

  • Biomedical imaging
  • Molecular probes
  • Cellular senescence

Background:

  • Cellular senescence detection is crucial for biological studies and clinical applications like cancer therapy monitoring.
  • Current imaging probes lack suitability for in vivo senescence detection.

Purpose of the Study:

  • To develop an activatable near-infrared (NIR) molecular probe for senescence detection.
  • To enable high-contrast NIR imaging of senescent cells in living subjects.

Main Methods:

  • Development of a novel activatable NIR molecular probe.
  • Probe designed for far-red excitation and NIR emission.
  • High "turn-on" ratio upon activation by senescence-associated β-galactosidase (SABG).

Main Results:

  • Successful demonstration of NIR imaging for DNA damage-induced senescence.
  • In vitro validation of the probe's efficacy.
  • In vivo imaging in human tumor xenograft models achieved.

Conclusions:

  • The developed NIR probe is effective for detecting cellular senescence.
  • This technology facilitates image-guided interventions and therapy monitoring.

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